| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.252315 |
| Chromosome: | chromosome 10 |
| Location: | 6149864 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g463900 | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAACTCAAATTGGGCAGTTCATTCTCTCA |
| Internal bar code: | GACTGCCTAGGTGTTCTACACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 126 |
| LEAP-Seq percent confirming: | 83.3784 |
| LEAP-Seq n confirming: | 1234 |
| LEAP-Seq n nonconfirming: | 246 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTAACATGCAGATGGCTGA |
| Suggested primer 2: | AGCAGAGGTTGTTGTCGCTT |