| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.252315 |
| Chromosome: | chromosome 10 |
| Location: | 6149865 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g463900 | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCAAGGGACACAGCTGGCACAACGCGAT |
| Internal bar code: | GCGGGGCATCTGTGGAGCGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 623 |
| LEAP-Seq percent confirming: | 98.6223 |
| LEAP-Seq n confirming: | 6371 |
| LEAP-Seq n nonconfirming: | 89 |
| LEAP-Seq n unique pos: | 91 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTAACATGCAGATGGCTGA |
| Suggested primer 2: | AGCAGAGGTTGTTGTCGCTT |