| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.252318 |
| Chromosome: | chromosome 6 |
| Location: | 1230605 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g257750 | (1 of 2) IPR023150 - Double Clp-N motif | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTTGTGAACGGTTACGGGGCTTTGGACT |
| Internal bar code: | GCCGTAGCGTTAGCCTACCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 276 |
| LEAP-Seq percent confirming: | 99.8434 |
| LEAP-Seq n confirming: | 5102 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTATGTGGTGCATGAGGTC |
| Suggested primer 2: | AGCTTCTACCAAGAGCTGCG |