| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.252320 |
| Chromosome: | chromosome 17 |
| Location: | 6200780 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g741900 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCTAGGACAGCAGGGTCGAAGAGTGCAG |
| Internal bar code: | CCCGGTTTCAAGTACTCATCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 913 |
| LEAP-Seq percent confirming: | 99.2818 |
| LEAP-Seq n confirming: | 18525 |
| LEAP-Seq n nonconfirming: | 134 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTATGCGATTTCTGTGCAGC |
| Suggested primer 2: | GCTGTCCAATTACGGCTGAT |