| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.252346 |
| Chromosome: | chromosome 7 |
| Location: | 176184 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g313350 | (1 of 3) PTHR22642:SF2 - AMIDOHYDROLASE FAMILY PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCAGCGGCGGTGGCGGCACTCATTTCATA |
| Internal bar code: | CCGTGCAGGACAACCGGCGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2 |
| LEAP-Seq percent confirming: | 73.4841 |
| LEAP-Seq n confirming: | 2145 |
| LEAP-Seq n nonconfirming: | 774 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGATCCACCCATCAGGAAG |
| Suggested primer 2: | GCTCATACGCTCACACAGGA |