| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.252378 |
| Chromosome: | chromosome 2 |
| Location: | 3723429 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095250 | (1 of 1) 6.6.1.2 - Cobaltochelatase / Hydrogenobyrinic acid a,c-diamide cobaltochelatase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCCTTCAGCGCGCTGCGGCTGAGGTCC |
| Internal bar code: | ATGTTGGCTGCGGTTAAATAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 439 |
| LEAP-Seq percent confirming: | 99.2908 |
| LEAP-Seq n confirming: | 700 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGTCAGATCCTCACGCAC |
| Suggested primer 2: | ACCAGCGTGCAGGACTACTT |