| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.252386 |
| Chromosome: | chromosome 13 |
| Location: | 712714 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g566500 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTTTTGGTTTTGGACCTGGGTTTGGAAC |
| Internal bar code: | GATGGGTCCTCAAGCACGATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 453 |
| LEAP-Seq percent confirming: | 66.3707 |
| LEAP-Seq n confirming: | 1719 |
| LEAP-Seq n nonconfirming: | 871 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGCAGTACCGGGCATCAG |
| Suggested primer 2: | AGTGGGATGGAGATGGAGG |