Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.252418 |
Chromosome: | chromosome 12 |
Location: | 1260392 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g489300 | (1 of 2) IPR000742//IPR001881//IPR009030 - EGF-like domain // EGF-like calcium-binding domain // Insulin-like growth factor binding protein, N-terminal | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTATCTCCTTTACACAGCGGTCCACGCACG |
Internal bar code: | CCTAGGCTGACGCATCTCATAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1205 |
LEAP-Seq percent confirming: | 99.628 |
LEAP-Seq n confirming: | 5357 |
LEAP-Seq n nonconfirming: | 20 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTGTCCTGTCGTACCTGCTG |
Suggested primer 2: | CATCGCAGAAAGGAGAGAGG |