| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.252445 |
| Chromosome: | chromosome 2 |
| Location: | 5129222 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g105150 | (1 of 1) 4.1.1.19 - Arginine decarboxylase / L-arginine carboxy-lyase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCTGGTGCACTCACATACGTCGCTGCAG |
| Internal bar code: | ACAACCAGCATGGCGTACTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 890 |
| LEAP-Seq percent confirming: | 99.7309 |
| LEAP-Seq n confirming: | 7783 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCGCCACTTCCTTTACTA |
| Suggested primer 2: | ACGCAAAGGACTCATGCTCT |