Insertion junction: LMJ.RY0402.252455_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.252455
Chromosome:chromosome 11
Location:1212015
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467695 (1 of 39) IPR009003 - Peptidase S1, PA clan 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGAGTGGCTTGTTCGCCGCTTCTTCCTTT
Internal bar code:CCGCTTGACCGTGTGGATGCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:580
LEAP-Seq percent confirming:97.3568
LEAP-Seq n confirming:884
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCTCGTGGATCAGTAGCA
Suggested primer 2:CATATGCCGGTCACTCACAC