Insertion junction: LMJ.RY0402.252461_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.252461
Chromosome:chromosome 12
Location:2790505
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g502800 (1 of 1) K14768 - U3 small nucleolar RNA-associated protein 7 (UTP7, WDR46) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTCGGCTTCCGGTCCTCGATGATGTTGC
Internal bar code:CACAAAGGCGTAAGACCGGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:552
LEAP-Seq percent confirming:99.3526
LEAP-Seq n confirming:2302
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCAGTGAAGACACTCCGA
Suggested primer 2:GTTGACCCTGCCTTCTCTTG