| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.252469 |
| Chromosome: | chromosome 1 |
| Location: | 2401871 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g013250 | (1 of 2) IPR006357//IPR023214 - HAD-superfamily hydrolase, subfamily IIA // HAD-like domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTCAGGCACAAGCTGGCGTGCACTGTCC |
| Internal bar code: | GTGTGGGATCCGCGCTGAGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 823 |
| LEAP-Seq percent confirming: | 98.9274 |
| LEAP-Seq n confirming: | 6087 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 98 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCTGCCTCTGTACACCAC |
| Suggested primer 2: | TAGAATCACCTGGGGGAGTG |