| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.252471 |
| Chromosome: | chromosome 1 |
| Location: | 3868581 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g025050 | (1 of 9) PTHR10071 - TRANSCRIPTION FACTOR GATA GATA BINDING FACTOR | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACTGATGCCGGTCCTGATGCTCTTCTCTC |
| Internal bar code: | GACTTCCTTTCTTAGGAACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 496 |
| LEAP-Seq percent confirming: | 98.6842 |
| LEAP-Seq n confirming: | 375 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAACGAGAAGAGGGCAAC |
| Suggested primer 2: | TTTGCGGATACCTAACCCAG |