| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.252476 |
| Chromosome: | chromosome 1 |
| Location: | 6954787 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g050150 | NFO1 | NADH:flavin oxidoreductase/NADH oxidase; (1 of 1) 1.6.99.1 - NADPH dehydrogenase / NADPH diaphorase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGTACGTCAAGGCCTGCTGTTTGCGTGT |
| Internal bar code: | TGCGGCTTATATTTAACTGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 501 |
| LEAP-Seq percent confirming: | 99.6287 |
| LEAP-Seq n confirming: | 1610 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAGATGACCGAGGCAGAC |
| Suggested primer 2: | TGGGAGCTATGACACAGCAG |