| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.252497 |
| Chromosome: | chromosome 10 |
| Location: | 5775290 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g461300 | 5'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCAAGGCTTCTGACTCCTATAATATAC |
| Internal bar code: | CACGACACATGGCCGGCGTACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 537 |
| LEAP-Seq percent confirming: | 95.6348 |
| LEAP-Seq n confirming: | 5499 |
| LEAP-Seq n nonconfirming: | 251 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTAGCAAGTAGGGTGAGG |
| Suggested primer 2: | AAATCGTCTACATCGGTGCC |