| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.252504 |
| Chromosome: | chromosome 3 |
| Location: | 3699795 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g169300 | (1 of 2) K05681 - ATP-binding cassette, subfamily G (WHITE), member 2 (ABCG2) | CDS|intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGAAGACCGGAACGCCGGCCCCGGAAG |
| Internal bar code: | GTTGCCGATTGTTATCGTACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1017 |
| LEAP-Seq percent confirming: | 99.6351 |
| LEAP-Seq n confirming: | 12014 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTAGAATCCGGCCTGAAG |
| Suggested primer 2: | CCAGGCATACACACGAACAC |