| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.252525 |
| Chromosome: | chromosome 3 |
| Location: | 7656068 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g204100 | ELG5 | Exostosin-like glycosyltransferase 5; (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACGACATGGACATTGTCGATGATGATGA |
| Internal bar code: | ATCCCAGCCGCCTCCGGTTACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 258 |
| LEAP-Seq percent confirming: | 36.5586 |
| LEAP-Seq n confirming: | 2131 |
| LEAP-Seq n nonconfirming: | 3698 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCACACACACATGCACAC |
| Suggested primer 2: | TTGGCCCCATGAAGTAAGTC |