| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.252570 |
| Chromosome: | chromosome 16 |
| Location: | 1899291 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g655900 | (1 of 5) IPR007213//IPR029063 - Leucine carboxyl methyltransferase // S-adenosyl-L-methionine-dependent methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGAGGATGACATTGTTTACTGTCTGCTG |
| Internal bar code: | CGGGCCCATTCGTCCATCATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 269 |
| LEAP-Seq percent confirming: | 99.3915 |
| LEAP-Seq n confirming: | 490 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTGCAAAACAGAGACGTG |
| Suggested primer 2: | CCTCACCTGACCTGCTTCTC |