| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.252578 |
| Chromosome: | chromosome 9 |
| Location: | 7242506 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g412550 | (1 of 3) IPR000104//IPR005069 - Antifreeze protein, type I // Nucleotide-diphospho-sugar transferase | 5'UTR|CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAAAGTTGAATTATTGCTGTTTGTGTTCA |
| Internal bar code: | TGTGCGGAATAATGCGGACGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 975 |
| LEAP-Seq percent confirming: | 98.3667 |
| LEAP-Seq n confirming: | 3493 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAGTCCACTCAACACAGT |
| Suggested primer 2: | CTGCTTAGGGTCTTTGCTGG |