Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.252599 |
Chromosome: | chromosome 2 |
Location: | 2721613 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g093650 | (1 of 1) 1.14.12.22 - Carbazole 1,9a-dioxygenase / CARDO | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGGCCCTGCAGCGGTCAGCAGCAACTGT |
Internal bar code: | CTCTGTCGGTTCTGGACTTTCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 485 |
LEAP-Seq percent confirming: | 98.5318 |
LEAP-Seq n confirming: | 604 |
LEAP-Seq n nonconfirming: | 9 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGGACTACGGGTGCATTCT |
Suggested primer 2: | GTGGACAAGTCCGCATACCT |