| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.252600 |
| Chromosome: | chromosome 3 |
| Location: | 5345225 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g184200 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCACAATTCTCTTATGTAGTTCTACGCCA |
| Internal bar code: | GCCTCAGGCGATAGGTCGGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 589 |
| LEAP-Seq percent confirming: | 99.7812 |
| LEAP-Seq n confirming: | 456 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGGCTGATCCCTGTTTCG |
| Suggested primer 2: | CTTGAAACCTGGGCCTATCA |