Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.252604 |
Chromosome: | chromosome_8 |
Location: | 988285 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g362000 | FAP72 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCAAACACGCGAGCACACGGGTACAATTAC |
Internal bar code: | GGACTGGTCCTGGTAAGTTCTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 595 |
LEAP-Seq percent confirming: | 99.6533 |
LEAP-Seq n confirming: | 1437 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAGAAGCAGCCTTCGAACAA |
Suggested primer 2: | GCGCGTATCCTTCAGTAAGC |