Insertion junction: LMJ.RY0402.252607_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.252607
Chromosome:chromosome 5
Location:3437377
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g241350 (1 of 1) 3.6.3.36 - Taurine-transporting ATPase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTCAAGTGAGTCCCGGCTTTCCCAGCCAC
Internal bar code:CTCAGATCGTGTCACAGTGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:247
LEAP-Seq percent confirming:99.2029
LEAP-Seq n confirming:2987
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CCACCCGCTTGTATCTTGTT
Suggested primer 2:CCTTGCTACGCCTTCACTTC