| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.252612 |
| Chromosome: | chromosome 14 |
| Location: | 3422832 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630600 | (1 of 12) IPR000104//IPR002893 - Antifreeze protein, type I // Zinc finger, MYND-type | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGACATTCAGTTTGCACGATTGCGTGGC |
| Internal bar code: | GCCGTCCGTGTCTCGCGCTACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 454 |
| LEAP-Seq percent confirming: | 99.308 |
| LEAP-Seq n confirming: | 861 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGATGGCTTCCCATTTTCA |
| Suggested primer 2: | GGCTGAGGCAGATGTACTCC |