| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.252641 |
| Chromosome: | chromosome_9 |
| Location: | 7032675 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g411200 | TEF5 | Rieske [2Fe-2S] domain containing protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGAATCGGGAACGGCCGGCTGTGAGCGTGG |
| Internal bar code: | GCCGGATCGCCCCCACGAACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 609 |
| LEAP-Seq percent confirming: | 98.5095 |
| LEAP-Seq n confirming: | 727 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCGTGAGGGTCTACACTG |
| Suggested primer 2: | TATGATAAGCGTGCGACTGC |