| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.252655 |
| Chromosome: | scaffold 18 |
| Location: | 191486 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g749547 | (1 of 1) PF07510 - Protein of unknown function (DUF1524) (DUF1524) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGTGGTGGGAATGGTGAGTTGGAATCGT |
| Internal bar code: | GCCAATCATGCAAATCTTGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 577 |
| LEAP-Seq percent confirming: | 98.9175 |
| LEAP-Seq n confirming: | 731 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGGTCACTTGCTGCTCCT |
| Suggested primer 2: | AAGGGGTAGTTGGCGAGATT |