| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.252655 |
| Chromosome: | scaffold 18 |
| Location: | 191487 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g749547 | (1 of 1) PF07510 - Protein of unknown function (DUF1524) (DUF1524) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCCCCAATTGAGTTGTTGTACGGCTGGC |
| Internal bar code: | ATGTCCGTTCATTCGGGTCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 737 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGGTCACTTGCTGCTCCT |
| Suggested primer 2: | AAGGGGTAGTTGGCGAGATT |