Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.252661 |
Chromosome: | chromosome 10 |
Location: | 1510477 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g428800 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCTCAGTTTCCCTTCCAACGTGCCACCA |
Internal bar code: | ACCCCATGTCTACGTCAGGGTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 578 |
LEAP-Seq percent confirming: | 99.4324 |
LEAP-Seq n confirming: | 4555 |
LEAP-Seq n nonconfirming: | 26 |
LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAGGACTGCTATGGTGCTGA |
Suggested primer 2: | TCAATTTTGTTGTGGGCAAA |