| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.252662 |
| Chromosome: | chromosome_14 |
| Location: | 1866727 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g620600 | PHC2 | Cell wall protein pherophorin-C2 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCATCTTGTAATGATCGACGGTCAAGCTGC |
| Internal bar code: | GGTTTCTGTTTGCCGTTTACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2757 |
| LEAP-Seq percent confirming: | 99.284 |
| LEAP-Seq n confirming: | 6379 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGTTGACACCCAGATCAA |
| Suggested primer 2: | CGCCAGTTACAGGGAATGTT |