Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.252694 |
Chromosome: | chromosome_10 |
Location: | 3990550 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g448850 | FAP135,ODA5AK,CAL5 | Calpain cystein protease and Flagellar Associated Protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGCAATGGACACCAGCCTGCCATGCAACCT |
Internal bar code: | TGCGCATCTAGCCCGCTCGAGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 622 |
LEAP-Seq percent confirming: | 98.0901 |
LEAP-Seq n confirming: | 4160 |
LEAP-Seq n nonconfirming: | 81 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTACGGTACATTACGCCGCT |
Suggested primer 2: | AAAGGAAGAAAGGAGCAGGG |