Insertion junction: LMJ.RY0402.252738_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.252738
Chromosome:chromosome 13
Location:1595077
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g573351 (1 of 1) K02960 - small subunit ribosomal protein S16e (RP-S16e, RPS16) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCACGAGCCGCGCACAAAGACACCCACT
Internal bar code:CATCGTCAGCAGCCAGACTGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:557
LEAP-Seq percent confirming:97.4181
LEAP-Seq n confirming:981
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCACTTCCACGACACAGT
Suggested primer 2:ATGCTCCCTACTGAAAGCGA