| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.252738 |
| Chromosome: | chromosome 13 |
| Location: | 1595078 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g573351 | (1 of 1) K02960 - small subunit ribosomal protein S16e (RP-S16e, RPS16) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCAGGTACACTGGCTGTGCGATGGGACA |
| Internal bar code: | AAGGTCCTGCCAGGTTGTAACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 621 |
| LEAP-Seq percent confirming: | 99.5567 |
| LEAP-Seq n confirming: | 1123 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCTCCCTACTGAAAGCGA |
| Suggested primer 2: | CTGCACTTCCACGACACAGT |