Insertion junction: LMJ.RY0402.252743_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.252743
Chromosome:chromosome 11
Location:3019184
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g478600 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGAAGCGACTGTGCGGCGGATGACGTGTA
Internal bar code:GCGCCTTAATACGTCGGCGGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:358
LEAP-Seq percent confirming:99.3667
LEAP-Seq n confirming:1569
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CCGCTCTACCTTGCTTGTTC
Suggested primer 2:GAGACCCAACAGAATCCGAA