| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.252750 |
| Chromosome: | chromosome 9 |
| Location: | 4894441 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397771 | OPR117 | OctotricoPeptide Repeat protein 117 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTTACGTGCGCATGTATGTTGCGTTGCG |
| Internal bar code: | TGCAGGGGACAGCCTGGGCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 575 |
| LEAP-Seq percent confirming: | 99.2405 |
| LEAP-Seq n confirming: | 392 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACTTCTACCGCATGCTCA |
| Suggested primer 2: | ACCCAATCCCGTAAGGAAAC |