Insertion junction: LMJ.RY0402.252788_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.252788
Chromosome:chromosome 14
Location:3373720
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g630200 FAP312 Flagellar Associated Protein 312 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCCCTCACCCCCCCCCCCAAAGCAAAGGC
Internal bar code:GCGCTGAACACGTTAAGGCGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:85
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CACAGAGCTCAGCCCACATA
Suggested primer 2:CCGATACCTGTGTGCGTATG