| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.252794 |
| Chromosome: | chromosome 17 |
| Location: | 4003348 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g728950 | RFD2 | diaminohydroxyphosphoribosylaminopyrimidine deaminase; (1 of 1) PTHR11079//PTHR11079:SF76 - CYTOSINE DEAMINASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTTCGCAAAGCTCCTCCGAAGCGGGGTC |
| Internal bar code: | TAAAGTGGCAATTAGATCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 453 |
| LEAP-Seq percent confirming: | 99.7743 |
| LEAP-Seq n confirming: | 884 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTTAAAGGCTGCGTGTGA |
| Suggested primer 2: | GGGCTGTCATAGAAAGCAGC |