| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.252802 |
| Chromosome: | chromosome 3 |
| Location: | 8898371 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g207825 | (1 of 1) K03241 - translation initiation factor eIF-2B subunit gamma (EIF2B3) | 3'UTR|outside_mRNA | |
| Cre03.g207937 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCACGGCATGGCACACGACGCGCCTGGT |
| Internal bar code: | AAGGCAACTGTCGCGGTCTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 487 |
| LEAP-Seq percent confirming: | 99.3808 |
| LEAP-Seq n confirming: | 963 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTTGACCAGGTATGCCGT |
| Suggested primer 2: | GGCTTCATGTGATATGGGCT |