Insertion junction: LMJ.RY0402.252804_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.252804
Chromosome:chromosome 2
Location:1704354
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g086000 ARS8 Arylsulfatase; (1 of 1) IPR002591//IPR003882//IPR017850 - Type I phosphodiesterase/nucleotide pyrophosphatase/phosphate transferase // Pistil-specific extensin-like protein // Alkaline-phosphatase-like, core domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCCGTTGCTTTACAGCGGATCTTTCTGTT
Internal bar code:GTGGAGGGAACATGGATGAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:642
LEAP-Seq percent confirming:99.3718
LEAP-Seq n confirming:4429
LEAP-Seq n nonconfirming:28
LEAP-Seq n unique pos:89

Suggested primers for confirmation by PCR

Suggested primer 1:TGACTCCCTGGGCACTATTC
Suggested primer 2:CTAGCTAGTGGCGGTGGAAG