| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.252804 |
| Chromosome: | chromosome 13 |
| Location: | 225973 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g563600 | UBQ7 | Ubiquitin-fusion protein; (1 of 5) IPR000626//IPR019956//IPR029071 - Ubiquitin domain // Ubiquitin // Ubiquitin-related domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAAAACTACGTACGGCATCCCGCGCTGA |
| Internal bar code: | AGCGTGTGGCAGTACTTGTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 775 |
| LEAP-Seq percent confirming: | 98.8645 |
| LEAP-Seq n confirming: | 3918 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTATTTTGTGCCCAAAGGC |
| Suggested primer 2: | ACTAGGCATTTGATGGGCAG |