| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.252815 |
| Chromosome: | chromosome 16 |
| Location: | 2909652 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g663850 | SSS5 | Soluble starch synthase V; (1 of 2) PTHR12526//PTHR12526:SF304 - GLYCOSYLTRANSFERASE // STARCH SYNTHASE 4, CHLOROPLASTIC/AMYLOPLASTIC-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTAAGCACCGCACGCGTGCGGGCCAGGC |
| Internal bar code: | CTCCTGTTATCTAATTATTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1195 |
| LEAP-Seq percent confirming: | 99.3703 |
| LEAP-Seq n confirming: | 1578 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGGTATGTCAGGTAGGAT |
| Suggested primer 2: | CCACGTCTGCAGTGATAGGA |