| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.252843 |
| Chromosome: | chromosome 14 |
| Location: | 226213 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g609202 | Conserved Protein With Tyrosinase Domain; (1 of 1) 3.4.24.7 - Interstitial collagenase / Vertebrate collagenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGCCCCCCCTCGTCGGCCTTCCTCCATG |
| Internal bar code: | GTAAAGCGTCGTTGCTAGGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 54 |
| LEAP-Seq percent confirming: | 99.6337 |
| LEAP-Seq n confirming: | 272 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAACAGGTCGACAGGACT |
| Suggested primer 2: | AGGAAGAGGCTTTGTGTCCA |