| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.252849 |
| Chromosome: | chromosome 10 |
| Location: | 4211334 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g450000 | (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTCTTAAGGTCCATCAATAGGCACCTGT |
| Internal bar code: | GAGCTTTGACCGCTAGGTGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 234 |
| LEAP-Seq percent confirming: | 98.9899 |
| LEAP-Seq n confirming: | 392 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCCTTAAGCAGCGGGAAC |
| Suggested primer 2: | GCACGCAAGTATGTGCATTT |