| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.252856 |
| Chromosome: | chromosome 2 |
| Location: | 6435125 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g115750 | GPD9 | (1 of 2) PTHR23344:SF28 - GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE 1; Glycerophosphoryl diester phosphodiesterase family protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGTATTTATACAAACTTTGCAGTGTTAA |
| Internal bar code: | GGGGATTAGGGGGCTCCGCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 552 |
| LEAP-Seq percent confirming: | 99.1039 |
| LEAP-Seq n confirming: | 553 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCACCGTCAACATAGTGC |
| Suggested primer 2: | GGGGTCAAAGTTGTTGTGCT |