| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.252875 |
| Chromosome: | chromosome 2 |
| Location: | 5732924 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g110250 | PCD2 | Polyketide cyclase/dehydrase; (1 of 2) PTHR24078//PTHR24078:SF290 - DNAJ HOMOLOG SUBFAMILY C MEMBER // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAGAAATGTGTGCCTGCGCCCCGCATGAG |
| Internal bar code: | TGGGCACTTGAGTTGCCTGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 974 |
| LEAP-Seq percent confirming: | 98.904 |
| LEAP-Seq n confirming: | 13356 |
| LEAP-Seq n nonconfirming: | 148 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTAGGTAGCGTTGTCGGCT |
| Suggested primer 2: | TTCGGCTTTGAGTGACAGTG |