| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.252880 |
| Chromosome: | chromosome 6 |
| Location: | 8908971 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g311200 | MFT21 | Major facilitator superfamily transporter; (1 of 3) PTHR23505//PTHR23505:SF12 - FAMILY NOT NAMED // MAJOR FACILITATOR PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGTCACTATTTCTGCCCCCTCACCTGCG |
| Internal bar code: | CTGAAAGTGCCCAGAGTGCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 296 |
| LEAP-Seq percent confirming: | 91.3447 |
| LEAP-Seq n confirming: | 591 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCTGTTGATGTTGCTGT |
| Suggested primer 2: | TGTGCTGAATCATGGAGAGC |