| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.252881 |
| Chromosome: | chromosome 9 |
| Location: | 3298170 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388171 | (1 of 2) PTHR12905//PTHR12905:SF0 - SPERM PROTEIN HOMOLOG // METALLOPHOSPHOESTERASE DOMAIN-CONTAINING PROTEIN 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGACCGATGCCGGGCGCTGCTGGGCGTGA |
| Internal bar code: | TCTCGGGCTGACGGATAAGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 721 |
| LEAP-Seq percent confirming: | 94.1341 |
| LEAP-Seq n confirming: | 337 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTCTCAAGCACTGCATCC |
| Suggested primer 2: | ACGCACAACGGACAATATGA |