| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.252905 |
| Chromosome: | chromosome 3 |
| Location: | 3840417 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g170650 | (1 of 1) IPR001849//IPR011993 - Pleckstrin homology domain // Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCATCTTGCTACTCCTCCTCTTCCCCCT |
| Internal bar code: | GGGCAGGGGTCGTACGGGCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 69 |
| LEAP-Seq percent confirming: | 99.337 |
| LEAP-Seq n confirming: | 899 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCCTTAGTAATGAGCCG |
| Suggested primer 2: | AATCGTTCCCTTGCTTTCCT |