| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.252905 |
| Chromosome: | chromosome 6 |
| Location: | 6353734 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g292050 | MMP21 | Matrix metalloproteinase; (1 of 40) 3.4.24.38 - Gametolysin / Lysin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAATGTGACAATGAAGAATTGAACTTCGG |
| Internal bar code: | AGCCAATGTGGCGGGCTGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 673 |
| LEAP-Seq percent confirming: | 98.4823 |
| LEAP-Seq n confirming: | 1752 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGCTTTCAACTCAGGCA |
| Suggested primer 2: | CACTAAAAGCAGTCCGGGTC |