| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.252939 |
| Chromosome: | chromosome 6 |
| Location: | 3058775 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g274500 | (1 of 1) K14396 - polyadenylate-binding protein 2 (PABPN1, PABP2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGTGAATAGAAAGTTGAGATGCGGCTGG |
| Internal bar code: | AGATCTTGGTTGGCGACGGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 548 |
| LEAP-Seq percent confirming: | 98.5667 |
| LEAP-Seq n confirming: | 1788 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGGCAACTTTATGGGAGG |
| Suggested primer 2: | CAGCGAGCCTCATATGTTCA |