Insertion junction: LMJ.RY0402.252945_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.252945
Chromosome:chromosome 1
Location:7457912
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g053550 (1 of 19) IPR000225//IPR016024 - Armadillo // Armadillo-type fold 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGCACGATCCGCATTGCCGTGGTCCATTA
Internal bar code:AGCCCCGTTGTTGTGCTTCATT

Confirmation - LEAP-Seq

LEAP-Seq distance:1246
LEAP-Seq percent confirming:99.4233
LEAP-Seq n confirming:30000
LEAP-Seq n nonconfirming:174
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:GGAATCAACCCAATACCGTG
Suggested primer 2:AGGCCATCAAGTGAGTCGTC